| Primer | Species | LP | RP | Tml | Tmr | Length | Score | Target | Senstive |
|---|---|---|---|---|---|---|---|---|---|
| Primer 221 | Methanosphaera cuniculi | CTGGGCAGATGTATCAGCAC | AGTGCTGTAACGCGTGCA | 58.41 | 60.28 | 162 | 94 |
100.00%
|
100.00%
|
| Primer 222 | Methanosphaera cuniculi | GGGCAGATGTATCAGCACAAC | AGTGCTGTAACGCGTGCA | 59.33 | 60.28 | 160 | 94 |
100.00%
|
100.00%
|
| Primer 223 | Methanosphaera cuniculi | CACAAACCTCTCTACGTGCTG | TGGGCTGTAGCTTGATTGCA | 58.94 | 59.96 | 166 | 94 |
100.00%
|
100.00%
|
| Primer 224 | Methanosphaera cuniculi | AGACTCGGTCCTCAACTTGC | TGGTCCCCAAGCAACCAT | 59.68 | 58.73 | 183 | 93 |
100.00%
|
100.00%
|
| Primer 225 | Methanosphaera cuniculi | AGCAAGCGAACTTAAATGGGG | TTTCCTGCACGAATGTTGGT | 59.18 | 58.31 | 177 | 93 |
100.00%
|
100.00%
|
| Primer 226 | Methanosphaera cuniculi | AAGGTGGAGCAAACGCTGT | GCATTCCCCAGTCGTCACA | 60.15 | 60.00 | 273 | 93 |
100.00%
|
100.00%
|
| Primer 227 | Methanosphaera cuniculi | GAAGGTGGAGCAAACGCTG | GCATTCCCCAGTCGTCACA | 59.42 | 60.00 | 274 | 93 |
100.00%
|
100.00%
|
| Primer 228 | Methanosphaera cuniculi | AGTGACAATGGCAGTATGGGC | ACAGCTCCTGGTGCTAGAAA | 60.68 | 58.65 | 209 | 93 |
100.00%
|
100.00%
|
| Primer 229 | Methanosphaera cuniculi | ACTGGGCAGATGTATCAGCAC | GTGCTGTAACGCGTGCAT | 60.13 | 59.14 | 162 | 93 |
100.00%
|
100.00%
|
| Primer 230 | Methanosphaera cuniculi | AGGACCACATGCATGTACTGG | GATGCCCTCAGCTTCCACA | 60.07 | 59.70 | 187 | 93 |
100.00%
|
100.00%
|